Combinatorial Immunophenotyping of Cell Populations with an Electronic Antibody Microarray

Immunophenotyping is widely used to characterize cell populations in basic research and to diagnose diseases from surface biomarkers in the clinic. This process usually requires complex instruments such as flow cytometers or fluorescence microscopes, which are typically housed in centralized laboratories. Microfluidics are combined with an integrated electrical sensor network to create an antibody microarray for label‐free cell immunophenotyping against multiple antigens. The device works by fractionating the sample via capturing target subpopulations in an array of microfluidic chambers functionalized against different antigens and by electrically quantifying the cell capture statistics through a network of code‐multiplexed electrical sensors. Through a combinatorial arrangement of antibody sequences along different microfluidic paths, the device can measure the prevalence of different cell subpopulations in a sample from computational analysis of the electrical output signal. The device performance is characterized by analyzing heterogeneous samples of mixed tumor cell populations and then the technique is applied to determine leukocyte subpopulations in blood samples and the results are validated against complete blood cell count and flow cytometry results. Label‐free immunophenotyping of cell populations against multiple targets on a disposable electronic chip presents opportunities in global health and telemedicine applications for cell‐based diagnostics and health monitoring.     

论三峡水库“蓄清排浑”运用方式及其优化

泥沙问题是三峡工程的关键技术问题之一,在三峡工程论证和初步设计阶段提出水库采取"蓄清排浑"的运用方式,可以解决泥沙问题。2003年水库蓄水运用以来的实践表明,水库基本遵循了"蓄清排浑"调度运用原则,并根据上游来水来沙减少等新情况对水库运用方式进行了优化调整。本文系统分析了三峡水库"蓄清排浑"运行方式及其优化调整的利弊,包括提前5年实施175 m试验性蓄水、汛期水位动态变化、汛末提前蓄水等对水库淤积和坝下游河道演变的影响,提出了进一步优化水库调度方式,形成"蓄清排浑"运用的新模式,保持水库长期使用的建议,试图为三峡水库科学高效安全运用提供科技支撑。     

重力注水过程流动不稳定现象关键影响因素研究

重力驱动注水过程中由于流量较小以及蒸汽的积聚可能导致流动不稳定现象的发生，对核反应堆安全运行具有重要的影响。通过实验研究的方法，搭建了重力注水模拟实验装置，研究了不同蒸汽出口形阻、高位储水箱水位和加热棒初始温度下流动不稳定现象的变化规律。结果表明，重力驱动注水过程流动不稳定现象包含冷却水初次注入阶段、注入水逐出阶段和冷却水再注入阶段等。在一定冷却水初始温度、冷却水入口形阻以及系统压力下，蒸汽排出速度以及实验本体内筒顶部的聚集情况取决于蒸汽出口形阻，减小蒸汽出口形阻可加快蒸汽排放速度，压力峰峰值降低、振荡周期变长，有利于系统稳定；提高高位储水箱水位加快了冷却水注入速率，增加了加热棒被淹没率，降低了流动不稳定现象的发生次数和持续时间；随加热棒初始温度的升高，冷却水流量出现了波动向停滞的转变，流动不稳定现象发生的次数增加且持续时间加长。     

高供氧压力下高马赫数氧枪数值模拟

  以北方某钢厂100 t转炉为原型，建立顶吹转炉炉内流场的三维数学模型，采用Fluent软件研究了不同高马赫数氧气射流与熔池钢液速度流场分布之间的依赖关系。研究发现，高马赫数氧枪在Ma（马赫数）为2.0～2.3时，曲线平稳，为最佳供氧压力。在提高供氧压力的同时，氧气射流的最大速度、熔池钢液面的冲击直径及冲击深度也随之增加。模拟结果显示，氧气射流在设计工况氧压小于1.0 MPa时，射流之间相互干扰作用最弱；氧气射流在设计工况氧压力大于1.0 MPa后，冲击直径与冲击深度增幅较小。基于上述研究，在实际生产中应用了高马赫数氧枪后，并结合变枪变压操作工艺，可以改善熔池底部钢液流动状况、稳定转炉吹炼过程、控制炉渣喷溅。     

基于协同优化算法的流速仪检定系统设计

提出了一种小型轻便的流速仪检定系统的设计方案,并运用改进的多学科协同优化方法求解各学科设计变量的最优解。首先介绍了算法思想及改进措施,并根据系统设计要求建立了各学科的设计变量、目标函数和约束条件；其次,运用遗传算法求解各学科设计变量的最优解,并根据优化结果完善了系统的设计方案,同时运用计算机仿真等方法验证了优化结果的可行性。结果表明,优化后的检定系统满足流体力学效应、匀速运动时间、系统重量等设计要求,同时证明了协同优化算法解决多学科设计优化问题的有效性。     

Modeling of Slug Velocity and Pressure Drop in Gas‐Liquid‐Liquid Slug Flow

Gas‐liquid‐liquid slug flow in a capillary reactor is a promising new concept that allows one to incorporate gas‐liquid reaction, liquid‐liquid extraction, and facile catalyst separation in a single unit. In order to assess the performance of a gas‐liquid‐liquid slug flow reactor, it is necessary to predict the slug velocity and pressure drop to ascertain residence times and reaction rates. New empirical models for velocity and pressure drop were developed based on existing models for two‐phase gas‐liquid and liquid‐liquid slug flows, and these were validated experimentally.     

Flow Compartments in Viscoelastic Fluids Using Radial Impellers in Stirred Tanks

The influence of viscoelastic flow properties on fluid dynamics using radial impellers is investigated. The use of transparent model fluids allows for the optical measurement of general flow behavior with a fluorescence dying technique. By varying viscoelastic flow properties, size of agitators and rotational frequency, the impact of these parameters on fluid dynamics is analyzed. Toroidally shaped, cavern‐like flow compartments form around the agitators in all fluids in specific rotational frequency ranges, preventing an efficient mixing. By balancing elastic with centrifugal forces, a simple model is developed with which compartment sizes can be predicted with good accuracy. The results indicate a good suitability of the elasticity number as a scale‐up criterion.     

The residual zonal flow in tokamak plasmas with a poloidal electric field

In a tokamak plasma with auxiliary heating by cyclotron waves, a poloidal electric field will be produced, and as a consequence influence the residual zonal flow(RZF) level. The poloidal electric field can also be induced through biasing electrodes at the edge region of tokamaks.Numerical evaluation for a large aspect ratio circular cross section tokamak for the electron cyclotron wave heating indicates that the RZF level decreases significantly when the poloidal electric field increases. Qualitatively, the ion cyclotron wave heating is able to increase the RZF level. It is difficult to apply the calculation to the real cyclotron wave heating experiments since we need to know factors such as the plasma profiles, the exact power deposition and the cross section geometry, etc. It is possible to use the cyclotron wave heating to control the zonal flow and then to control the turbulence level in tokamak experiments.     

Imaging the Curie Point isothermal surface and predicting its impact on the geothermal regime within the Nile Delta,Egypt

The available aeromagnetic data together with information from some available wells have been used in the current study to shed the light on the geothermal setup of the Nile Delta Province. The aeromagnetic data was reduced-to-pole and critically analyzed using the Spectral Analysis Technique through the Fast Fourier Transform (FFT), to determine the expected depths to the Basement and Curie Point (CPD) surfaces. The geothermal gradient between CPD and earth's surface was estimated, and accordingly the heat flow was evaluated using the proposed thermal conductivity. Then, the Basement Surface Temperature (BST) was estimated, by which the geothermal gradient and heat flow for the igneous-rocky basement and sedimentary-rocky horizon was independently predicted. By which, it could be possible to distinguish between the heat flow contribution from each horizon separately.